Intergrated Phamacology

1.Does scopolamine produce a cognitive defect as demonstrated by significant changes in the performance of animals in the water maze? 2.If there is an effect, is this changed by the concurrent administration of the anticholinesterases neostigmine or physostigmine? a) Collect the path length data for 6 rats per treatment over 4 days. There are four treatments in all. Enter your raw data into an Excel spreadsheet and calculate t-test P values for each day for each treatment group compared with its appropriate control(s) using Excel’s two sample t-test assuming equal variances. Make a note of the means of your data sets +SEMs (standard errors of the mean), and the P values, and present this data inthree tables. b) Plot the means of your data onto a total of twoline graphs. Graphs should be of path length vs time (i.e. day number).One graph should show scopolamine data vs vehicle 1 data (i.e two sets of data) over 4 days. The other graph should show the effects of concurrent administration of each anticholinesterase with scopolamine, compared to scopolamine alone (three sets of data). For each data point add SEM error bars and asterisks denoting statistical significance. c) Give a summary of your findings by answering points (1) and (2) above, and justify your answers using your statistical data judiciously (max. 400 words total). Your answer should include a comment on the probable pharmacological mechanisms involved.